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Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane. Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.
The dye inserts itself between the double helical structure of nucleic acids, allowing for visualization of the molecules under UV light. [6] EtBr has absorbance maxima at 300-360 nm and fluorescent emission maxima at 500-590 nm, with the detection limit of 0.5-5.0 ng/band. [ 6 ]
Resist for deep-UV, e-beam, x-ray; Resin itself is DUV sensitive (slow) ... /PAG blends; Photocrosslinking photoresist is a type of photoresist, which could crosslink ...
When using the fluorescent protein dye trichloroethanol, a subsequent protein staining is omitted if it was added to the gel solution and the gel was irradiated with UV light after electrophoresis. [39] [40] In Coomassie staining, gel is fixed in a 50% ethanol 10% glacial acetic acid solution for 1 hr. Then the solution is changed for fresh one ...
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Nd:YAG lasers, mainly via their second and third harmonics, are widely used to excite dye lasers either in the liquid [44] or solid state. [45] They are also used as pump sources for vibronically broadened solid-state lasers such as Cr 4+ :YAG or via the second harmonic for pumping Ti:sapphire lasers .
Nylon 46 (nylon 4-6, nylon 4/6 or nylon 4,6, PA46, Polyamide 46) is a high heat resistant polyamide or nylon. Envalior (formerly DSM ) is the only commercial supplier of this resin, which markets under the trade name Stanyl.
An agarose gel cast in tray, to be used for gel electrophoresis. Agarose gel is a three-dimensional matrix formed of helical agarose molecules in supercoiled bundles that are aggregated into three-dimensional structures with channels and pores through which biomolecules can pass. [3]