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Note:If MAST is combining reverse complement DNA strands, the position p-value is not corrected for multiple tests. Sequence p-value: The p-value of a match of a sequence to a motif is defined as the probability of a randomly generated sequence of the same length having a match score at least as large as the largest match score of any position ...
The 5 prime portion of the RC probe contains the reverse complement sequence of the desired target specific primer sequence. In RC-PCR, no target specific primers are present in the reaction mixture. Instead target specific primers are formed as the reaction proceeds. A typical reaction employing the approach requires four oligonucleotides. The ...
Reverse Complement will be used in situations where the complements may need to be reversed in a sequence. [6] Search Menu consists of Find, Find Next, Find Previous, Next N, Find in File, and DO BLAST Search. The Find, Find Next, and Find Previous are used to find occurrences in certain sections of a query sequence.
EMBOSS is a free c software analysis package developed for the needs of the molecular biology and bioinformatics user community. [1] The software automatically copes with data in a variety of formats and even allows transparent retrieval of sequence data from the web.
Sequerome has the following features: profiling Sequence alignment reports from BLAST by linking the results page to a panel of third party services, tabbed browsing allowing user to come back earlier operations, visit third party services to perform customized sequence manipulations, one-box any-format sequence input and alternate options for sequence input including visiting third party ...
Product 1 derives from strand 1's which have a unique tag sequence (called α in Figure 2) next to the Illumina adapter 1 and product 2 has a unique tag (called β in Figure 2) next to the Illumina adapter 1. (In each strand, tag α is the reverse complement of tag β and vice versa).
The key idea of rna22 is that the reverse complement of any salient sequence features that one can identify in mature microRNA sequences (using pattern discovery techniques) should allow one to identify candidate microRNA target sites in a sequence of interest: rna22 makes use of the Teiresias algorithm to discover such salient features. Once a ...
Velvet is an algorithm package that has been designed to deal with de novo genome assembly and short read sequencing alignments.This is achieved through the manipulation of de Bruijn graphs for genomic sequence assembly via the removal of errors and the simplification of repeated regions. [2]