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10x Genomics was founded in 2012 by Serge Saxonov, Ben Hindson and Kevin Ness to create advanced testing equipment for use in cellular biology. [3] Prior to starting the company, Saxonov was the founding architect, and director of research and development at 23andMe. [2]
MGISEQ-2000: 375M FCS flow cell, 1500M FCL flow cell per flow cell. 1 to 9 days depending on instrument, read length and number of flow cells run at a time. $5– $120 Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp: 99.9%: 1.2 to 1.4 billion: 1 to 2 weeks: $60–130: Low cost per base. Slower than other methods.
The 10X Genomics Visium assay is a newer and improved version of the Spatial Transcriptomics assay. It also utilizes spotted arrays of mRNA-capturing probes on the surface of glass slides but with increased spot number, minimized spot size and increased amount of capture probes per spot.
Previous efforts of coupling index-sorting measurements from single cell sorts with scRNA-seq were limited to running a small sample size and were not compatible with multiplexing and massive parallel high-throughput sequencing. CITE-seq has been shown to be compatible with high-throughput microfluidic platforms like 10X Genomics and Drop-seq ...
Following this lawsuit, 10x Genomics discontinued their linked-read assay. [15] An exception was made for linked-read products which had already been sold by the company prior to the lawsuit, allowing 10x Genomics to continue to provide those researchers with services such as support and warranty maintenance for this technology. [citation needed]
Time per run 2 hours 24 hours 3–10 days 7–14 days 2–4 hours 20 minutes - 3 hours 3–5 days Read length 200-400 bp 700 bp 100x100 bp paired end 50x50 bp paired end 14,000 bp 400-900 bp 100/150/200 bp paired end Cost per run US$350 US$7,000 US$6,000 (30x human genome) US$4,000 $125–300 USD US$4 (single read/reaction) N/A Cost per Mb
Anne Wojcicki wanted to run things—alone. It was 2009 and her startup 23andMe was riding high, fueled by a steady stream of venture money, intriguing personal accounts of genome exploration, and ...
In fact, 500 to 10,000 cells are captured per sample from a single cell suspension. The protocol is performed easily and allows a high cell recovery rate of up to 65%. The global workflow of the droplet-based platform takes 8 hours and so is faster than the Microwell-based method (BD Rhapsody), which takes 10 hours.