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In organic chemistry, Fehling's solution is a chemical reagent used to differentiate between water-soluble carbohydrate and ketone (>C=O) functional groups, and as a test for reducing sugars and non-reducing sugars, supplementary to the Tollens' reagent test. The test was developed by German chemist Hermann von Fehling in 1849. [1]
Alpha-hydroxy ketones are also called acyloins. [1] They are commonly formed by condensation or reductive coupling of two carbonyl (C=O) compounds or oxidation of ketones. The simplest such compound is hydroxyacetone. If the alcohol is primary, alpha-hydroxy ketones give a positive Fehling's test. Beta-hydroxy ketones are a type of aldol.
Ketones give positive results in Brady's test, the reaction with 2,4-dinitrophenylhydrazine to give the corresponding hydrazone. Ketones may be distinguished from aldehydes by giving a negative result with Tollens' reagent or with Fehling's solution. Methyl ketones give positive results for the iodoform test. [7] Ketones also give positive ...
Another test relies on reaction of the furfural with phloroglucinol to produce a colored compound with high molar absorptivity. [4] It also gives a positive test with hydrazines, hydrazones, α-hydroxy ketones and 1,2-dicarbonyls. Both Tollens' reagent and Fehling's reagent give positive results with formic acid. [citation needed]
Fehling's solution was used for many years as a diagnostic test for diabetes, a disease in which blood glucose levels are dangerously elevated by a failure to produce enough insulin (type 1 diabetes) or by an inability to respond to insulin (type 2 diabetes). Measuring the amount of oxidizing agent (in this case, Fehling's solution) reduced by ...
A new approach to a routine blood test could predict a person’s 30-year risk of heart disease, research published Saturday in the New England Journal of Medicine found.. Doctors have long ...