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Focus stacking – also called focal plane merging, z-stacking, [1] or focus blending – is a digital image processing technique which combines multiple images taken at different focus distances to give a resulting image with a greater depth of field (DOF) than any of the individual source images.
If the sample is larger than the field of view of the image sensor, the sample also has to be shifted laterally. An alternative approach is to move the image plane through the sample to create the image stack. [32] Long experiments can be carried out, for example with stacks recorded every 10 sec–10 min over the timespan of days.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Simultaneous two-color label-free stimulated Raman scattering z-stack imaging of mouse ear (red: protein, green: lipid, image is 220 by 220 microns the total depth is 60 microns, the pixel dwell time is 2 microsecond).
For example, 50 Cron uniquely identifies the Summicron-M 50 mm f /2 construction, although the exact version is not specified. Many Leica M lenses went through ...
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2006 - Leica Photogrammetry Suite 9.1 (new TIN format: LTF, terrain split and merge improvements) 2008 - Leica Photogrammetry Suite 9.2 (ATE improvements, new sensor models, enhanced mosaicking) 2008 - With the re-branding of "Leica Geosystems Geospatial Imaging" to "ERDAS", the "Leica Photogrammetry Suite" name was converted to an acronym, "LPS".
The brand Leica, better known for Leica Cameras, was formed on 2 April 1990 after the merger of several companies.In 2005, the company was acquired by Hexagon AB.Leica was listed on the Swiss stock exchange until the 7 August 2006 which saw the cancellation of all publicly held registered shares in Leica Geosystems Holdings Ltd and delisting of all listed registered shares of a par value of ...