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Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. [2] Spectrophotometry uses photometers , known as spectrophotometers, that can measure the intensity of a light beam at different wavelengths.
A spectrophotometer is able to determine the average concentrations of the nucleic acids DNA or RNA present in a mixture, as well as their purity. Spectrophotometric analysis is based on the principles that nucleic acids absorb ultraviolet light in a specific pattern.
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
Specialized fluorescent dyes bind specifically to the substances of interest. A spectrophotometer is used in this method to measure the natural absorbance of light at 260 nm (for DNA and RNA) or 280 nm (for proteins). [5] [6] [7] [8]
An XPS spectrometer. A spectrometer (/ s p ɛ k ˈ t r ɒ m ɪ t ər /) is a scientific instrument used to separate and measure spectral components of a physical phenomenon. Spectrometer is a broad term often used to describe instruments that measure a continuous variable of a phenomenon where the spectral components are somehow mixed.
How to Reset Female Hormones For Weight Loss. This article was reviewed by Craig Primack, MD, FACP, FAAP, MFOMA. Hormones can be helpful heroes, supporting the immune system and a healthy sleep ...