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Blood is normally sterile. [1] The presence of bacteria in the blood is termed bacteremia, and the presence of fungi is called fungemia. [2] Minor damage to the skin [3] or mucous membranes, which can occur in situations like toothbrushing or defecation, [4] [5] can introduce bacteria into the bloodstream, but this bacteremia is normally transient and is rarely detected in cultures because the ...
The blood culture bottle contains transport media to preserve any microorganisms present while they are being transported to the laboratory for cultures. Because it is unknown whether the pathogens are anaerobic (living without oxygen) or aerobic (living with oxygen), blood is collected to test for both. The aerobic bottle is filled first, and ...
Phlebotomy is the process of making a puncture in a vein, usually in the arm, with a cannula for the purpose of drawing blood. [1] The procedure itself is known as a venipuncture, which is also used for intravenous therapy. A person who performs a phlebotomy is called a phlebotomist, although most doctors, nurses, and other technicians can also ...
Two sets of blood cultures (aerobic and anaerobic) are recommended without delaying the initiation of antibiotics. Cultures from other sites such as respiratory secretions, urine, wounds, cerebrospinal fluid, and catheter insertion sites (in-situ more than 48 hours) are recommended if infections from these sites are suspected. [5]
Tube cap color or type in order of draw Additive Usage and comments Blood culture bottle: Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms: Usually drawn first for minimal risk of contamination. Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms. Blue
Vacutainer. A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing.