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Schematic of cell adhesion. Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released ...
Scratch wound healing assay experiment of rhabdomyosarcoma, a cancer cell line. A wound healing assay is a laboratory technique used to study cell migration and cell–cell interaction. This is also called a scratch assay because it is done by making a scratch on a cell monolayer and capturing images at regular intervals by time lapse ...
It is a versatile tool in understanding the mechanisms of proliferation, adhesion, and metastasis of cancer cells. Parallel plate flow chambers are widely used also for drug testing in the cellular chemotaxis assay [9] and for novel targeted drug delivery systems based on leukocyte-endothelium adhesion processes.
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate derived cells (with the exception of hematopoietic cells) can be cultured and require a 2 dimensional monolayer that to facilitate cell adhesion and spreading. [2]
Meanwhile, other groups have demonstrated the requirement for cell-ECM adhesion proteins or their regulators in MDCK branching morphogenesis. [32] [33] Using a modified protocol for MDCK cell culture and branching morphogenesis, Gierke and Wittman established the requirement for microtubule dynamics in regulating the early steps in branching. [34]
The process is highly regulated by cell adhesion molecules, particularly, the addressin also known as MADCAM1. This antigen is known for its role in tissue-specific adhesion of lymphocytes to high endothelium venules. [23] Through these interactions they play a crucial role in orchestrating circulating lymphocytes.