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Saturation mutagenesis, or site saturation mutagenesis (SSM), or simply site saturation, is a random mutagenesis technique used in protein engineering, in which a single codon or set of codons is substituted with all possible amino acids at the position. [1]
Genetic saturation is the result of multiple substitutions at the same site in a sequence, or identical substitutions in different sequences, such that the apparent sequence divergence rate is lower than the actual divergence that has occurred. [1]
Protein-ligand binding typically changes the structure of the target protein, thereby changing its function in a cell. The distinction between the two Hill equations is whether they measure occupancy or response. The Hill equation reflects the occupancy of macromolecules: the fraction that is saturated or bound by the ligand.
Saturation (magnetic), the state when a magnetic material is fully magnetized; Saturated fluid or saturated vapor, contains as much thermal energy as it can without boiling or condensing Saturated steam; Dew point, which is a temperature that occurs when atmospheric relative humidity reaches 100% and the air is saturated with moisture
Site saturation mutagenesis is a type of site-directed mutagenesis. This image shows the saturation mutagenesis of a single position in a theoretical 10-residue protein. The wild type version of the protein is shown at the top, with M representing the first amino acid methionine, and * representing the termination of translation.
Nutrition (Per 3-ounce serving): Calories: 170 Fat: 8 g (Saturated Fat: 2 g) Sodium: 40 mg Carbs: 0 g (Fiber: 0 g, Sugar: 0 g) Protein: 23 g. American Tuna was born in 2005 out of a collaboration ...
Protein is important at breakfast because it keeps you satisfied throughout the morning. However, there are some options you should skip. ... (or no) saturated fat, which supports heart health. In ...
The fractional saturation Y O2 of the myoglobin is what proportion of the total myoglobin concentration is made up of oxygen-bound myoglobin, which can be rearranged as the concentration of free oxygen over the sum of that concentration and the dissociation constant K. Since diatomic oxygen is a gas, its concentration in solution can be thought ...