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The FOX reagent, or ferrous oxidation−xylenol orange, is used to measure levels of hydrogen peroxide in biological systems. [1] The reagent is incubated with the sample and absorbance of the product form after a series of oxidation reactions is then measured at a wavelength of 560 nm.
Hydrogen peroxide is a chemical compound with the formula H 2 O 2.In its pure form, it is a very pale blue [5] liquid that is slightly more viscous than water.It is used as an oxidizer, bleaching agent, and antiseptic, usually as a dilute solution (3%–6% by weight) in water for consumer use and in higher concentrations for industrial use.
Permanganometry is one of the techniques used in chemical quantitative analysis. It is a redox titration that involves the use of permanganates to measure the amount of analyte present in unknown chemical samples. [1]
The free radicals generated by this process engage in secondary reactions. For example, the hydroxyl is a powerful, non-selective oxidant. [6] Oxidation of an organic compound by Fenton's reagent is rapid and exothermic and results in the oxidation of contaminants to primarily carbon dioxide and water.
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (). [1]
1 part of aqueous H 2 O 2 (hydrogen peroxide, 30%) at 75 or 80 °C [1] typically for 10 minutes. This base-peroxide mixture removes organic residues. Particles are also very effectively removed, even insoluble particles, since SC-1 modifies the surface and particle zeta potentials and causes them to repel. [4]
This method starts with a solution of hydrogen peroxide and sulfuric acid. To this a solution containing potassium iodide, sodium thiosulfate, and starch is added. There are two reactions occurring simultaneously in the solution. In the first, slow reaction, iodine is produced: H 2 O 2 + 2 I − + 2 H + → I 2 + 2 H 2 O
For example, the loss of blue color in leuco-methylene blue is selective for hydrogen peroxide. [21] Quantitative analysis of hydroperoxides can be performed using potentiometric titration with lithium aluminium hydride. [22] Another way to evaluate the content of peracids and peroxides is the volumetric titration with alkoxides such as sodium ...