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  2. Colony-forming unit - Wikipedia

    en.wikipedia.org/wiki/Colony-forming_unit

    A dilution made with bacteria and peptoned water is placed in an Agar plate (Agar plate count for food samples or Trypticase soy agar for clinic samples) and spread over the plate by tipping in the pattern shown.

  3. Plate count agar - Wikipedia

    en.wikipedia.org/wiki/Plate_count_agar

    The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.

  4. Agar plate - Wikipedia

    en.wikipedia.org/wiki/Agar_plate

    An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...

  5. Miles and Misra method - Wikipedia

    en.wikipedia.org/wiki/Miles_and_Misra_method

    Bacterial suspension or homogenate. Method. The inoculum / suspension is serially diluted by adding 1x of suspension to 9x of diluent. When the quantity of bacteria is unknown, dilutions should be made to at least 10 −8. Three plates are needed for each dilution series, for statistical reasons an average of at least 3 counts are needed.

  6. Bacteriological water analysis - Wikipedia

    en.wikipedia.org/wiki/Bacteriological_water_analysis

    The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. To be effective, the dilution of the original sample must be arranged so that on average between 30 and 300 colonies of the target bacterium are grown.

  7. Total viable count - Wikipedia

    en.wikipedia.org/wiki/Total_Viable_Count

    The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate

  8. Colonial morphology - Wikipedia

    en.wikipedia.org/wiki/Colonial_morphology

    [1]: 167–8 Bacteria that produce capsules often have a slimy (mucoid) consistency. [2]: 495 When certain microorganisms are grown on blood agar, they may digest the blood in the medium, causing visible hemolysis (destruction of red blood cells) on the agar plate. In colonial morphology, hemolysis is classified into three types: alpha-, beta ...

  9. Agar dilution - Wikipedia

    en.wikipedia.org/wiki/Agar_dilution

    After this, bacteria prepared to a standard concentration are added as a spot to each plate, with 10 4 colony forming units (CFU) per spot. This technique allows for replicate spots of one bacterial type to be tested [3] or spots of different bacteria so that the MIC of the antibiotic against multiple types of bacteria can be tested. Necessary ...