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The observer then perceives a cyan (or magenta) square on the blank sheet. This complementary color afterimage is more easily explained by the trichromatic color theory (Young–Helmholtz theory) than the traditional RYB color theory; in the opponent-process theory, fatigue of pathways promoting red produces the illusion of a cyan square. [39]
In this traditional scheme, a complementary color pair contains one primary color (yellow, blue or red) and a secondary color (green, purple or orange). The complement of any primary color can be made by combining the two other primary colors. For example, to achieve the complement of yellow (a primary color) one could combine red and blue.
For example, to see "stygian blue": staring at bright yellow causes a dark blue afterimage, then on looking at black, the blue is seen as blue against the black, also as dark as the black. The color is not possible to achieve through normal vision, because the lack of incident light (in the black) prevents saturation of the blue/yellow ...
For the example color of S 2030-Y90R, the saturation is calculated as = / = / = / = NCS lightness (v) is a color's perceptual characteristic to contain more of the achromatic elementary colors black or white than another color. NCS lightness values varies from 0 for the elementary color black (S) to 1 for the elementary color white (W).
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.