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Vibrio cholerae is a species of Gram-negative, facultative anaerobe and comma-shaped bacteria. [1] The bacteria naturally live in brackish or saltwater where they attach themselves easily to the chitin -containing shells of crabs, shrimp, and other shellfish.
Vibrio spp. are facultative anaerobes that test positive for oxidase and do not form spores. [4] [5] All members of the genus are motile. They are able to have polar or lateral flagellum with or without sheaths. [4] [6] Vibrio species typically possess two chromosomes, which is unusual for bacteria.
Thiosulfate–citrate–bile salts–sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. [1] TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS agar, other rapid testing ...
Inhabitants of fresh or salt water, several species are pathogenic, including the type species Vibrio cholerae, which is the agent responsible for cholera. Most bioluminescent bacteria belong to this family, and are typically found as symbionts of deep-sea animals. [1]
The organism on the right produced no color, so it does not appear to be a lactose fermenter. MacConkey agar is a selective and differential culture medium for bacteria . It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation . [ 1 ]
Vibrio vulnificus is a species of Gram-negative, motile, curved rod-shaped (vibrio), pathogenic bacteria of the genus Vibrio. Present in marine environments such as estuaries, brackish ponds, or coastal areas, V. vulnificus is related to V. cholerae, the causative agent of cholera. [3] At least one strain of V. vulnificus is bioluminescent. [4]
Endo agar contains peptone, lactose, dipotassium phosphate, agar, sodium sulfite, basic fuchsin and was originally developed for the isolation of Salmonella typhi, but is now commonly used in water analysis. As in MacConkey agar, coliform organisms ferment the lactose, and the colonies become red.
When some otherwise non-hemolytic bacteria, such as strains of the cholera-causing bacteria Vibrio cholerae, are plated on blood agar, no clearings are observed surrounding isolated colonies, but the blood surrounding larger areas of growth turns green. This process, called hemodigestion, is caused by the metabolic by-products of the bacteria ...