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The extinction law's primary application is in chemical analysis, where it underlies the Beer–Lambert law, commonly called Beer's law. Beer's law states that a beam of visible light passing through a chemical solution of fixed geometry experiences absorption proportional to the solute concentration .
Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.
The absorbance of a material that has only one absorbing species also depends on the pathlength and the concentration of the species, according to the Beer–Lambert law =, where ε is the molar absorption coefficient of that material; c is the molar concentration of those species; ℓ is the path length.
To normalize the concentration to a 10mm equivalent, the following is done: 0.6 OD X (10/3) * 50 μg/mL=100 μg/mL Most spectrophotometers allow selection of the nucleic acid type and path length such that resultant concentration is normalized to the 10 mm path length which is based on the principles of Beer's law.
The technique makes use of the atomic absorption spectrum of a sample in order to assess the concentration of specific analytes within it. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the Beer–Lambert law.
In physics, the attenuation length or absorption length is the distance λ into a material when the probability has dropped to 1/e that a particle has not been absorbed. ...
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The analytical (total) concentration of a reactant R at the i th titration point is given by = + [] + where R 0 is the initial amount of R in the titration vessel, v 0 is the initial volume, [R] is the concentration of R in the burette and v i is the volume added. The burette concentration of a reactant not present in the burette is taken to be ...