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It is also used as an anticoagulant for laboratory testing, in that blood samples are collected into sodium citrate-containing tubes for tests such as the PT (INR), APTT, and fibrinogen levels. Sodium citrate is used in medical contexts as an alkalinizing agent in place of sodium bicarbonate, [2] to neutralize excess acid in the blood and urine ...
Blood is drawn into a test tube containing liquid sodium citrate, which acts as an anticoagulant by binding the calcium in a sample. The blood is mixed, then centrifuged to separate blood cells from plasma (as prothrombin time is most commonly measured using blood plasma). In newborns, a capillary whole blood specimen is used. [2]
Blue Top Vacutainer tube (sodium citrate vial) used for PT and PTT blood tests. Activated partial thromboplastin time (APTT) is typically analyzed by a medical technologist or laboratory technician, either manually or using an automated instrument at 37°C, which approximates normal human body temperature.
It binds calcium but not as strongly as EDTA. The correct proportion of this anticoagulant to blood is crucial because of the dilution, which can be reversed with the addition of calcium. Formulations include plain sodium citrate, acid-citrate-dextrose, and more. Oxalate has a mechanism similar to that of citrate. It is the anticoagulant used ...
Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms. [2] Blue ("light blue") Sodium citrate (weak calcium chelator/anticoagulant) Coagulation tests such as prothrombin time (PT) and partial thromboplastin time (PTT) and thrombin time (TT). Tube must be filled to the proper line. Plain red
A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing.
A second sample run with a different anticoagulant such as sodium citrate (blue top tube) to confirm the finding of pseudothrombocytopenia may be requested if there are doubts or concerns. [7] Other alternative anticoagulants are sodium fluoride , CPT ( trisodium citrate , pyridoxal 5′-phosphate and tris ), CTAD (citrate, theophylline ...
As a result, blood can now be stored for much longer, up to 21 days. [4] ACD was developed into CPD (citrate-phosphate-dextrose) in 1957, [5] a version with phosphate added intended to reduce phosphate leakage from red blood cells. It does not improve shelf life appreciably, but patient recovery is improved.