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The formation of methylene blue after the reaction of hydrogen sulfide with dimethyl-p-phenylenediamine and iron(III) at pH 0.4 – 0.7 is used to determine by photometric measurements sulfide concentration in the range 0.020 to 1.50 mg/L (20 ppb to 1.5 ppm). [62]
The aqueous solution in the classical reaction contains glucose, sodium hydroxide and methylene blue. [14] In the first step an acyloin of glucose is formed. The next step is a redox reaction of the acyloin with methylene blue in which the glucose is oxidized to diketone in alkaline solution [6] and methylene blue is reduced to colorless leucomethylene blue.
Gross pathology of a normal brain and a brain of a patient treated with methylene blue before death in a case of vasoplegic syndrome. Vasoplegic syndrome or vasoplegia syndrome (VPS) is a postperfusion syndrome characterized by low systemic vascular resistance and a high cardiac output.
Methylene blue is given as a 1% solution (10 mg/ml) 1 to 2 mg/kg administered intravenously slowly over five minutes. Although the response is usually rapid, the dose may be repeated in one hour if the level of methemoglobin is still high one hour after the initial infusion. Methylene blue inhibits monoamine oxidase, and serotonin toxicity can ...
Matilda Moldenhauer Brooks (1888–1981) was a cellular biologist best known for her 1932 discovery that the staining compound methylene blue is an antidote to carbon monoxide and cyanide poisoning. She held a PhD in zoology and spent her professional career working as a researcher at the United States Public Health Service and the University ...
(Vital stains include dyes like trypan blue and propidium iodide, which are either too bulky or too charged to cross the cell membrane, or which are actively rapidly pumped out by live cells.) Supravital staining can be combined with cell surface antibody staining (immunofluorescence) for applications such as FACS analysis. [4]
Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells). Those that enter and stain living cells are called supravital stains (e.g. New Methylene Blue and brilliant cresyl blue for reticulocyte staining). However, these stains are ...
After incubation, methylene blue is added. If anti-Toxo antibodies are present in the serum, the antibody-antigen complex activates complement to lyse the parasite membrane, Toxoplasma trophozoites are not stained (positive result); if there are no antibodies, trophozoites with intact membrane are stained and appear blue under microscope ...