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Pine Island Center is located at the intersection of Pine Island and Stringfellow Roads. Pine Island Road (County Road 78) is the only road that leads to the mainland. Pine Island Center is the location of the island's two large grocery stores, along with an elementary school, library, museum, swimming pool, and a large park.
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The St. James City CDP occupies the southern half of Pine Island, with the town center situated at the southern end of the island. The community is bordered by San Carlos Bay to the south, by Pine Island Sound to the west, by San Carlos Bay, Matlacha Pass, and Pine Island Creek to the east, and by the community of Pine Island Center to the north.
The Pine Island National Wildlife Refuge is part of the United States National Wildlife Refuge System, located on the southwest coast of Florida south of Charlotte Harbor, north of Sanibel Island in Pine Island Sound on La Costa Island. The 601-acre (2.43 km 2) refuge was established on September 15, 1908.