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Ultramicrobacteria are bacteria that are smaller than 0.1 μm 3 under all growth conditions. [1] [2] [3] This term was coined in 1981, describing cocci in seawater that were less than 0.3 μm in diameter. [4] Ultramicrobacteria have also been recovered from soil and appear to be a mixture of gram-positive, gram-negative and cell-wall-lacking ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Microcarrier cell culture, however, was the breakthrough required for cell culture to reach industrial and clinical significance. [2] Studies have shown that microcarrier suspensions, compared to multi-layer vessel culture, improve cell yield by 80-fold at only ten percent of Good Manufacturing Practice space, and only sixty percent of the ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Experiments generally start after 24 hours of incubation. Using living cells or tissue from the same organism are still considered to be ex vivo. One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted on the CAM membrane of a chicken embryo outside the organism (chicken).
The first report of minicells in the scientific literature dates to 1930., [3] but the first use of the name "minicell" dates to 1967 [2] Minicells of a variety of gram negative [4] and gram positive [5] [6] bacteria, including Escherichia coli [7] and Salmonella enterica, [8] have been reported, but in principle, minicells could be generated for any bacterial species that can be genetically ...
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
Bacterial morphological plasticity refers to changes in the shape and size that bacterial cells undergo when they encounter stressful environments. Although bacteria have evolved complex molecular strategies to maintain their shape, many are able to alter their shape as a survival strategy in response to protist predators, antibiotics, the immune response, and other threats.