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A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens requiring a defined biosafety level.
A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4).
BSL-4 cabinets and "Suit Laboratories" have special engineering and design features to prevent hazardous microorganisms from being disseminated into the outside environment. These biosafety suites , where PPPSs are used, are suites of laboratory rooms which are essentially equivalent to large Class III biosafety cabinets in which the interiors ...
Biosafety level designations are based on a composite of the design features, construction, containment facilities, equipment, practices and operational procedures required for working with agents from the various risk groups. [10]
The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). In the United States, the Centers for Disease Control and Prevention (CDC) have specified these levels. [8] In the European Union, the same biosafety levels are defined in a directive. [9]
A PAPR, gown, and biosafety cabinet in use in a BSL-3 laboratory. All parts of the PAPR are visible: the waist unit holding the fan, filter, and battery; the hose; and the mask, in this case a flexible, loose-fitting one. A powered air-purifying respirator (PAPR) is a type of respirator used to safeguard workers against contaminated air.