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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
The Cary 14 spectrophotometer was first produced in 1954 by the Applied Physics Corporation, which later was named the Cary Instruments Corporation after co-founder Howard Cary. [1] The instrument was a successor to the Cary 11, which was the first commercially available recording UV/Vis spectrophotometer. [3]
It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.
Infrared spectrophotometers were better suited than UV–Vis spectrophotometers to the analysis of C 4 hydrocarbons, particularly for applications in petroleum refining and gasoline production. [ 2 ] : 159 [ 4 ] : 17
In the field of photochemical analysis, it is common to measure the extinction of chemicals with high precision, often at the sub-nanometer scale, using UV/Vis spectroscopy. To understand fundamental relationships between a chemical's absorbance and its photoreactivity, a detailed analysis of the reactivity at a similar level of resolution is ...
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