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Using passive agglutination, Kunin detected the O-antigen found in the lipopolysaccharide (LPS) of E. coli. During the experiments, Kunin noticed that there was cross-reactivity between the rabbit antisera and many of the E. coli strains. One of the sera, O14, reacted to an antigen found in a large range of E. coli strains. Notably, the antigen ...
E. coli is a gram-negative, facultative anaerobe, nonsporulating coliform bacterium. [18] Cells are typically rod-shaped, and are about 2.0 μm long and 0.25–1.0 μm in diameter, with a cell volume of 0.6–0.7 μm 3. [19] [20] [21] E. coli stains gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane.
Referred to as E. coli O157:H7 or Shiga toxin-producing E. coli (STEC), this strain of E. coli can be particularly dangerous and even life-threatening. The primary sources of STEC outbreaks are ...
E. coli bacteria often carry multiple drug resistance plasmids, and under stress, readily transfer those plasmids to other species. Mixing of species in the intestines allows E. coli to accept and transfer plasmids from and to other bacteria. Thus, E. coli and the other enterobacteria are important reservoirs of transferable antibiotic ...
Media formulations containing the media supplement B27 (supplied by Invitrogen) are often erroneously referred to as chemically defined media (e.g. Yao et al., 2006) despite this product containing bovine serum albumin (Chen et al., 2008) using the above definitions this type of media is referred to as serum-free media.
One way E. coli is spread is when feces contaminate food and water. Since animals can potentially carry pathogenic E. coli, touching animals, such as at a petting zoo, can potentially put you at risk.
LPS is the most abundant antigen on the cell surface of most gram-negative bacteria, contributing up to 80% of the outer membrane of E. coli and Salmonella. [2] LPS increases the negative charge of the cell membrane and helps stabilize the overall membrane structure. It is of crucial importance to many gram-negative bacteria, which die if the ...
E. coli have 187 possible O antigens (6 later removed from list, 3 actually producing no LPS), [17] 53 H antigens, [18] and at least 72 K antigens. [19] Among these three, the O antigen has the best correlation with lineages; as a result, the O antigen is used to define the "serogroup" and is also used to define strains in taxonomy and ...