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Ultramicrobacteria are bacteria that are smaller than 0.1 μm 3 under all growth conditions. [1] [2] [3] This term was coined in 1981, describing cocci in seawater that were less than 0.3 μm in diameter. [4] Ultramicrobacteria have also been recovered from soil and appear to be a mixture of gram-positive, gram-negative and cell-wall-lacking ...
These cell types can be subcultured by simply taking a small volume of the parent culture and diluting it in fresh growth medium. Cell density in these cultures is normally measured in cells per milliliter for large eukaryotic cells, or as optical density for 600nm light for smaller cells like bacteria. The cells will often have a preferred ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
The culture of viruses requires the culture of cells of mammalian, plant, fungal or bacterial origin as hosts for the growth and replication of the virus. Whole wild type viruses, recombinant viruses or viral products may be generated in cell types other than their natural hosts under the right conditions.
Experiments generally start after 24 hours of incubation. Using living cells or tissue from the same organism are still considered to be ex vivo. One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted on the CAM membrane of a chicken embryo outside the organism (chicken).
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. [1]
Lancaster and Knoblich [4] define an organoid as a collection of organ-specific cell types that develops from stem cells or organ progenitors, self-organizes through cell sorting and spatially restricted lineage commitment in a manner similar to in vivo, and exhibits the following properties:
In comparison to batch culture, bacteria are maintained in exponential growth phase, and the growth rate of the bacteria is known. Related devices include turbidostats and auxostats . When Escherichia coli is growing very slowly with a doubling time of 16 hours in a chemostat most cells have a single chromosome.