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Molecular breeding is the application of molecular biology tools, often in plant breeding [1] [2] and animal breeding. [3] [4] In the broad sense, molecular breeding can be defined as the use of genetic manipulation performed at the level of DNA to improve traits of interest in plants and animals, and it may also include genetic engineering or gene manipulation, molecular marker-assisted ...
Marker assisted selection or marker aided selection (MAS) is an indirect selection process where a trait of interest is selected based on a marker (morphological, biochemical or DNA/RNA variation) linked to a trait of interest (e.g. productivity, disease resistance, abiotic stress tolerance, and quality), rather than on the trait itself.
For example, DArT has been used to conduct genome-wide analysis among Musa species, which includes bananas and plantains, which led to the development of a phylogenetic cladogram based on genetic markers derived from DArT techniques. These developments enhance breeding knowledge to obtain desirable yields and products.
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Human-directed genetic manipulation began with the domestication of plants and animals through artificial selection in about 12,000 BC. [1]: 1 Various techniques were developed to aid in breeding and selection. Hybridization was one way rapid changes in an organism's genetic makeup could be introduced. Crop hybridization most likely first ...
Plant breeders use different methods depending on the mode of reproduction of crops, which include: Self-fertilization, where pollen from a plant will fertilise reproductive cells or ovules of the same plant; Cross-pollination, where pollen from one plant can only fertilize a different plant
The main difference between DNA shuffling with restriction enzymes and molecular breeding is molecular breeding relies on the homology of the sequences for the annealing of the strands and PCR for extension whereas by using restriction enzymes, fragment ends that can be ligated are created. [1]
A key event in the conservation of plant genetic resources was the establishment of the International Board for Plant Genetic Resources (IBPGR) (now Bioversity International) in 1974, whose mandate was to promote and assist in the worldwide effort to collect and conserve the plant germplasm needed for future research and production. IBPGR ...