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The swab is then streaked on a culture medium. The culture is incubated at 37 °C (98.6 °F) for several days, to allow development of yeast or bacterial colonies. The characteristics (such as morphology and colour) of the colonies may allow initial diagnosis of the organism causing disease symptoms. [60]
At 37 °C (99 °F) either in the laboratory or in host tissue, S. schenckii assumes its yeast form. Macroscopically, the yeast form grows as smooth white or off-white colonies. Microscopically, yeast cells are 2 to 6 μm long and show an elongated cigar-shaped morphology. [1]
Antibiotics promote yeast (fungal) infections, including gastrointestinal (GI) Candida overgrowth and penetration of the GI mucosa. [9] While women are more susceptible to genital yeast infections, men can also be infected. Certain factors, such as prolonged antibiotic use, increase the risk for both men and women.
Candida albicans is an opportunistic pathogenic yeast [5] that is a common member of the human gut flora.It can also survive outside the human body. [6] [7] It is detected in the gastrointestinal tract and mouth in 40–60% of healthy adults.
A. thermomutatus has been cultivated on both Czapek yeast extract agar (CYA) plates and Malt Extract Agar Oxoid® (MEAOX) plates. The growth morphology of the colonies can be seen in the pictures below.
Some terms used to describe colonial morphology. When a specimen arrives in the microbiology laboratory, it is inoculated into an agar plate and placed in an incubator to encourage microbial growth. Because the appearance of microbial colonies changes as they grow, colonial morphology is examined at a specific time after the plate is inoculated.
The colony surface is smooth and has a pasty texture. [1] [10] There is considerable cell and colony morphology when S. salmonicolor is grown in culture. [6] The budding yeast-like cells produced during the asexual stage are ellipsoidal to subcylindrical and 8–25 × 2–5.5 μm. They can occur singly or in pairs.
The mycelial and yeast phases differ in their morphology, biochemistry, and ultrastructure. [8] The yeast form contains large amounts of α-(1,3)-linked glucan. [10] [page needed] [11] The chitin content of the mycelial form is greater than that of the yeast form, but the lipid content of both phases is comparable. [10] [page needed]