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At 37 °C (99 °F) either in the laboratory or in host tissue, S. schenckii assumes its yeast form. Macroscopically, the yeast form grows as smooth white or off-white colonies. Microscopically, yeast cells are 2 to 6 μm long and show an elongated cigar-shaped morphology. [1]
The swab is then streaked on a culture medium. The culture is incubated at 37 °C (98.6 °F) for several days, to allow development of yeast or bacterial colonies. The characteristics (such as morphology and colour) of the colonies may allow initial diagnosis of the organism causing disease symptoms. [60]
Antibiotics promote yeast (fungal) infections, including gastrointestinal (GI) Candida overgrowth and penetration of the GI mucosa. [9] While women are more susceptible to genital yeast infections, men can also be infected. Certain factors, such as prolonged antibiotic use, increase the risk for both men and women.
While Candida albicans is the most common yeast species associated with vaginal thrush, infection by other types of yeast can produce similar symptoms. A Hungarian study of 370 patients with confirmed vaginal yeast infections identified the following types of infection: [18] Candida albicans: 85.7%; Non-albicans Candida (8 species): 13.2%
Colonies of C. tropicalis on a Petri dish.. C. tropicalis is a vegetative cell [10] with the shape from round to oval ranging from approximately 2 – 10 micrometers. [3] A mould exhibits dimorphism [8] forming a single-celled yeast or so-called blastoconidia which reproduces by simple budding. [8]
Candida albicans is an opportunistic pathogenic yeast [5] that is a common member of the human gut flora.It can also survive outside the human body. [6] [7] It is detected in the gastrointestinal tract and mouth in 40–60% of healthy adults.
Colonies of K. marxianus are cream to brown in colour with the occasional pink pigmentation due to production of the iron chelate pigment, pulcherrimin. [ 10 ] [ 11 ] When grown on Wickerham's Yeast-Mold (YM) agar, the yeast cells appear globose, ellipsoidal or cylindrical, 2–6 x 3–11 μm in size. [ 10 ]
The colony surface is smooth and has a pasty texture. [1] [10] There is considerable cell and colony morphology when S. salmonicolor is grown in culture. [6] The budding yeast-like cells produced during the asexual stage are ellipsoidal to subcylindrical and 8–25 × 2–5.5 μm. They can occur singly or in pairs.