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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
1846: Carl Zeiss founded Carl Zeiss AG, to mass-produce microscopes and other optical instruments. 1850s: John Leonard Riddell, Professor of Chemistry at Tulane University, invents the first practical binocular microscope. [13] 1863: Henry Clifton Sorby develops a metallurgical microscope to observe structure of meteorites.
First workshop of Carl Zeiss in the center of Jena, c. 1847 Carl Zeiss Jena (1910) One of the Stasi's cameras with the special SO-3.5.1 (5/17mm) lens developed by Carl Zeiss, a so-called "needle eye lens", for shooting through keyholes or holes down to 1 mm in diameter 2 historical lenses of Carl Zeiss, Nr. 145077 and Nr. 145078, Tessar 1:4,5 F=5,5cm DRP 142294 (produced before 1910) Carl ...
The first combination of a confocal microscope with specialized computer hardware that enabled digital image processing was presented by Van der Voort et al. (1985). In the late 1980s, Biorad introduced the first commercial laser scanning confocal microscope to the market, followed by Carl Zeiss and Leica soon after. All of these instruments ...
Microscope by Carl Zeiss (1879) with optics by Abbe The resolution limit formula engraved in an Ernst Abbe memorial in Jena, Germany German stamp of 1968 Ernst Abbe, relief at his grave In 1866, he became a research director at the Zeiss Optical Works , and in 1868 he invented the apochromatic lens , a microscope lens which eliminates both the ...
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
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