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These pipettes commonly come in 5, 10, 25, and 50 mL volumes. A variety of propipetters [clarification needed] have been developed, both entirely manual and electrically assisted. Originally pipettes were made of soda-lime glass, but currently many are made of borosilicate glass; disposable graduated pipettes are often made of polystyrene.
[2] [3] They thus are used for smaller-scale transfers than equipment such as graduated pipettes, which come in 5, 10, 25 and 50 mL volumes. The most common type of pipettes can be set to a certain volume within its operational range and are called adjustable. These pipettes commonly have a label with their volume range like "10–100 μL".
These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 20, 50, and 100 mL. Volumetric pipettes are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration.
The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette has a tolerance of ±0.05 mL. (These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of ...
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. [1] These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes. [ 2 ]
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