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  2. Immunoassay - Wikipedia

    en.wikipedia.org/wiki/Immunoassay

    In immunology the particular macromolecule bound by an antibody is referred to as an antigen and the area on an antigen to which the antibody binds is called an epitope. In some cases, an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution.

  3. Fluorescence polarization immunoassay - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_polarization...

    The tracer and the specific antigen will compete to bind to the antibody and if the antigen is low in concentration, more tracer will be bound to the antibody resulting in a higher fluorescence polarization and vice versa. [7] A conventional FPIA follows the procedure below: A specific quantity of sample is added to reaction buffer.

  4. Latex fixation test - Wikipedia

    en.wikipedia.org/wiki/Latex_fixation_test

    A latex fixation test, also called a latex agglutination assay or test (LA assay or test), is an assay used clinically in the identification and typing of many important microorganisms. These tests use the patient's antigen - antibody immune response.

  5. Heterophile antibody test - Wikipedia

    en.wikipedia.org/wiki/Heterophile_antibody_test

    It is an improvement on the Paul–Bunnell test. [2] The test is specific for heterophile antibodies produced by the human immune system in response to EBV infection. Commercially available test kits are 70–92% sensitive and 96–100% specific, with a lower sensitivity in the first two weeks after clinical symptoms begin. [3] [4]

  6. Mass spectrometric immunoassay - Wikipedia

    en.wikipedia.org/wiki/Mass_spectrometric_immunoassay

    Mass spectrometric immunoassay (MSIA) is a rapid method is used to detect and/ or quantify antigens and or antibody analytes. [1] This method uses an analyte affinity (either through antigens or antibodies) isolation to extract targeted molecules and internal standards from biological fluid in preparation for matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI ...

  7. Direct fluorescent antibody - Wikipedia

    en.wikipedia.org/wiki/Direct_fluorescent_antibody

    Its name derives from the fact that it directly tests the presence of an antigen with the tagged antibody, unlike western blotting, which uses an indirect method of detection, where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondary antibody.

  8. Immunocytochemistry - Wikipedia

    en.wikipedia.org/wiki/Immunocytochemistry

    Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells.

  9. Complement fixation test - Wikipedia

    en.wikipedia.org/wiki/Complement_fixation_test

    The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs. It was widely used to diagnose infections, particularly with microbes that are not easily detected by culture methods, and in ...

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