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Bromothymol is used in obstetrics for detecting premature rupture of membranes. [11] Amniotic fluid typically has a pH > 7.2, bromothymol will therefore turn blue when brought in contact with fluid leaking from the amnion. As vaginal pH normally is acidic, the blue color indicates the presence of amniotic fluid.
A roll of universal indicator pape Colors of universal indicator. A universal indicator is a pH indicator made of a solution of several compounds that exhibit various smooth colour changes over a wide range pH values to indicate the acidity or alkalinity of solutions. A universal indicator can be in paper form or present in a form of a solution.
Low pH color Transition low end Transition high end High pH color Gentian violet (Methyl violet 10B) [4] yellow 0.0 2.0 blue-violet Malachite green (first transition) yellow 0.0 2.0 green Malachite green (second transition) green 11.6 14.0 colorless Thymol blue (first transition) red 1.2 2.8 yellow Thymol blue (second transition) yellow 8.0 9.6 ...
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Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
Phthalein dyes are a class of dyes mainly used as pH indicators, due to their ability to change colors depending on pH. [1] They are formed by the reaction of phthalic anhydride with various phenols. They are a subclass of triarylmethane dyes. Common phthalein dyes include: Bromothymol blue; Bromocresol green; Bromocresol purple; Cresol Red; o ...
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The agar contains citrate and ammonium ions (nitrogen source) and bromothymol blue (BTB) as a pH indicator. [2] Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized.