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Mannitol salt agar (MSA) is a selective, differential, and indicator medium used to isolate and identify Staphylococcus aureus from the clinical specimen. As its name suggests, mannitol salt agar (MSA) contains 1% mannitol (sugar), 7.5% salt, and agar as a solidifying agent.
Interpreting Results. Picture 1: Mannitol salt agar test as a part of a selective medium. On the left side grows Staphylococcus aureus and no growth took side on the right side. Image Source: ytimg.com. Picture 2: Mannitol salt agar test on three plates with corresponding results. Image Source: wp.com.
Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others.
An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Mannitol Salt Agar (MSA)- Composition, Principle, Preparation, Results, Uses. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens.
Mannitol salt agar (MSA) is both a selective and differential medium used in the isolation of staphylococci. It contains 7.5% sodium chloride and thus selects for those bacteria which can tolerate high salt concentrations. MSA also distinguishes bacteria based on the ability to ferment the sugar mannitol, the only carbohydrate in the medium. Theory
How to interpret results on MSA agar plate? While testing the organisms on MSA agar, we generally encounter three types of results. The color of the plate will either turn yellow, pink, or remains red.
Latest audio book. What is Mannitol Salt Agar (MSA)? Purpose of Mannitol Salt Agar (MSA) Principle of Mannitol Salt Agar (MSA) Composition of Mannitol Salt Agar (MSA) Preparation of Mannitol Salt Agar (MSA) Test Procedure on Mannitol Salt Agar (MSA) Results and Colony Characteristics in Mannitol Salt Agar (MSA) Quality Control on Mannitol Salt Agar
Mannitol Salt Agar (MSA) can be used to presumptively isolate and identify Staphylococci from human samples. Refer to the compositions of MSA and MacConkey agar below. MSA contains 75 g/L NaCl (7.5%) compared to the 5 g/L found in TSA and other all-purpose media.
Mannitol Salt Agar (MSA) is a selective and differential culture medium used for the isolation and identification of staphylococci. This agar is named after its main components: mannitol, sodium chloride, and agar.