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More elaborate incubators can also include the ability to lower the temperature (via refrigeration), or the ability to control humidity or CO 2 levels. This is important in the cultivation of mammalian cells, where the relative humidity is typically >80% to prevent evaporation and a slightly acidic pH is achieved by maintaining a CO 2 level of 5%.
An incubator is a device simulating avian incubation by keeping eggs warm at a particular temperature range and in the correct humidity with a turning mechanism to hatch them. The common names of the incubator in other terms include breeding / hatching machines or hatchers , setters , and egg breeding / equipment .
They need to be kept at body temperature (37 °C) in an incubator. [1] These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich medium that supplies the essential nutrients ( amino acids , carbohydrates , vitamins , minerals ), growth factors , hormones , and gases ( CO 2 , O 2 ), and ...
Ventilation guidelines are based on the minimum ventilation rate required to maintain acceptable levels of effluents. Carbon dioxide is used as a reference point, as it is the gas of highest emission at a relatively constant value of 0.005 L/s.
A thermostat exerts control by switching heating or cooling devices on or off, or by regulating the flow of a heat transfer fluid as needed, to maintain the correct temperature. A thermostat can often be the main control unit for a heating or cooling system, in applications ranging from ambient air control to automotive coolant control.
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The wet-bulb temperature is the lowest temperature that may be achieved by evaporative cooling of a water-wetted, ventilated surface.. By contrast, the dew point is the temperature to which the ambient air must be cooled to reach 100% relative humidity assuming there is no further evaporation into the air; it is the temperature where condensation (dew) and clouds would form.
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