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Saline solution for irrigation. Normal saline (NSS, NS or N/S) is the commonly used phrase for a solution of 0.90% w/v of NaCl, 308 mOsm/L or 9.0 g per liter. Less commonly, this solution is referred to as physiological saline or isotonic saline (because it is approximately isotonic to blood serum, which makes it a physiologically normal solution).
Saline solution normally contains 0.9% sodium chloride. This article walks you through application instructions based on the intended use.
The following is a sample recipe for BBS: 10 mM Sodium borate; 150 mM NaCl; Adjust pH to pH 8.2 The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use borate buffered saline solution upon dissolution in 500 ml of deionized water.
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
Saline solution is also sometimes used. [2] The U.S. Centers for Disease Control and Prevention has a fact sheet recommending one of four methods to make the water safe: [8] Boil: Use water that has been previously boiled for 1 minute and left to cool. At elevations above 6,500 feet, boil for 3 minutes.
The following is a sample recipe for TBST: 20 mM Tris; 150 mM NaCl; 0.1% Tween 20; Adjust pH with HCl to pH 7.4–7.6 The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water. [1]