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conjugative - mediate DNA transfer through conjugation and therefore spread rapidly among the bacterial cells of a population; e.g., F plasmid, many R and some col plasmids. nonconjugative - do not mediate DNA through conjugation, e.g., many R and col plasmids. The pBR322 plasmid is one of the first plasmids widely used as a cloning vector.
Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information and references, including lists of commonly used vector backbones, popular lentiviral ...
Download as PDF; Printable version; In other projects ... VectorDB was a database of sequence information for common vectors ... Plasmid; References. a b External ...
A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors . Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco , it was named after Francisco Bolivar Zapata , the postdoctoral researcher ...
The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector contains features that allow for the convenient insertion of a DNA fragment into the vector or its removal from the vector, for example through the presence of restriction sites.
P1 vectors also contain a P1 plasmid replicon, which ensures only one copy of the vector is present in a cell. However, there is a second P1 replicon- called the P1 lytic replicon- that is controlled by an inducible promoter. This promoter allows the amplification of more than one copy of the vector per cell prior to DNA extraction. [2] bac vector
Vector map of pUC19. pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. [2]
The term plasmid was coined in 1952 by the American molecular biologist Joshua Lederberg to refer to "any extrachromosomal hereditary determinant." [11] [12] The term's early usage included any bacterial genetic material that exists extrachromosomally for at least part of its replication cycle, but because that description includes bacterial viruses, the notion of plasmid was refined over time ...