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Stopped-flow spectrophotometers may function as stand-alone instruments, but they are often integrated into systems for circular dichroism (CD), absorbance, and/or fluorescence measurements, or equipped with various accessories to support specialized applications. Common stopped-flow accessories include:
Schematic diagram of the arrangement of optical components in a typical Spectrofluorometer. A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
This process enables it to transform the fluorescence data into a quantified concentration measurement. The device uses this established relationship to accurately determine the concentration of a sample. A specific instance of this technology is the Qubit 2.0 fluorometer, which is often used in conjunction with the "dsDNA BR Assay Kit."
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
Multicolor fluorescence image of living HeLa cells Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy , imaging probes, and spectroscopy .
The fictive model structure for this discussion has two confined quantized electronic and two hole subbands, e 1, e 2 and h 1, h 2, respectively. The linear absorption spectrum of such a structure shows the exciton resonances of the first (e1h1) and the second quantum well subbands (e 2 , h 2 ), as well as the absorption from the corresponding ...
Also, y-components to the excitation and fluorescence sine waves will be modulated, and lifetime can be determined from the modulation ratio of these y-components. Hence, 2 values for the lifetime can be determined from the phase-modulation method. The lifetimes are determined through a fitting procedures of these experimental parameters.