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Early in the development of microbiology, milk was used as a convenient, rich growth medium for propagating bacteria. The litmus in the medium acts as both a pH indicator and a redox (oxidation-reduction) indicator. The test itself tells whether the bacterium can ferment lactose, reduce litmus, form clots, form gas, or start peptonization. [2]
The fairly similar Babcock test is used primarily in the United States, although the Gerber method also enjoys significant use in the U.S. as well. [3] The Gerber method was developed and patented by Dr. Niklaus Gerber of Switzerland in 1891. [4] Milk fat is separated from proteins by adding sulfuric acid.
Litmus test may refer to: Litmus test (chemistry), used to determine the acidity of a chemical solution; Litmus test (politics), a question that seeks to find the character of a potential candidate by measuring a single indicator; The Litmus Test, a Radio 4 programme presented by Fred Harris; The Litmus Test, a 2004 album by Cut Chemist
Litmus is a water-soluble mixture of different dyes extracted from lichens. It is often absorbed onto filter paper to produce one of the oldest forms of pH indicator, used to test materials for acidity. In an acidic medium, blue litmus paper turns red, while in a basic or alkaline medium, red litmus paper turns blue. In short, it is a dye and ...
In this test, resazurin is added as a violet redox dye which turns mauvish-pink due to conversion to resorufin and then to colourless dihydroresorufin. This happens due to lowering of the oxidation-reduction potential in the milk sample caused by presence of bacteria which utilize available oxygen present in the milk for aerobic respiration .
The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds). [19] After decolorization, the gram-positive cell remains purple and the gram-negative cell loses its purple color. [19]
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It tests positive for the oxidase test, and is also a nonsaccharolytic bacterial species. Heat-stable lipases and proteases are produced by P. fluorescens and other similar pseudomonads. [4] These enzymes cause milk to spoil, by causing bitterness, casein breakdown, and ropiness due to production of slime and coagulation of proteins. [5] [6]