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  2. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2] Gram ...

  3. Gram-positive bacteria - Wikipedia

    en.wikipedia.org/wiki/Gram-positive_bacteria

    Both gram-positive and gram-negative bacteria commonly have a surface layer called an S-layer. In gram-positive bacteria, the S-layer is attached to the peptidoglycan layer. Gram-negative bacteria's S-layer is attached directly to the outer membrane. Specific to gram-positive bacteria is the presence of teichoic acids in the cell wall. Some of ...

  4. Cell envelope - Wikipedia

    en.wikipedia.org/wiki/Cell_envelope

    In Gram-negative bacteria an outer membrane is also included. [1] This envelope is not present in the Mollicutes where the cell wall is absent. Bacterial cell envelopes fall into two major categories: a Gram-positive type which stains purple during Gram staining and a Gram-negative type which

  5. Isolation (microbiology) - Wikipedia

    en.wikipedia.org/wiki/Isolation_(microbiology)

    Gram-negative bacteria will stain a pink color due to the thin layer of peptidoglycan. If a bacteria stains purple, due to the thick layer of peptidoglycan, the bacteria is a gram-positive bacteria. [4] In clinical microbiology numerous other staining techniques for particular organisms are used (acid fast bacterial stain for mycobacteria).

  6. Group A streptococcal infection - Wikipedia

    en.wikipedia.org/wiki/Group_A_streptococcal...

    A Gram stain is performed to show Gram-positive cocci in chains. Then, the organism is cultured on blood agar. The rapid pyrrolidonyl arylamidase (PYR) test is commonly used, wherein a positive reaction confers a presumptive identification of group A beta-hemolytic streptococci if the appearance and clinical context is consistent.

  7. Staphylococcus saprophyticus - Wikipedia

    en.wikipedia.org/wiki/Staphylococcus_saprophyticus

    Staphylococcus saprophyticus; S. saprophyticus on Mueller–Hinton agar exhibiting resistance to novobiocin characteristic for species identification : Gram stain of a Gram positive S. saprophyticus sample suspension, lab cultured on Tryptic Soy agar (TSA)

  8. Staphylococcus carnosus - Wikipedia

    en.wikipedia.org/wiki/Staphylococcus_carnosus

    Staphylococcus carnosus is a bacterium from the genus Staphylococcus that is both Gram-positive and coagulase-negative. [1] It was originally identified in dry sausage and is an important starter culture for meat fermentation.

  9. Mycobacterium leprae - Wikipedia

    en.wikipedia.org/wiki/Mycobacterium_leprae

    It is Gram-positive by Gram staining, but Mycobacterium leprae was traditionally stained with carbol fuchsin in the Ziehl–Neelsen stain. Because the bacilli are less acid-fast than Mycobacterium tuberculosis (MTB), the Fite-Faraco staining method, which has a lower acid concentration, is used now. [9] [10] In size and shape, it closely ...