Search results
Results From The WOW.Com Content Network
Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani , intended LB to stand for lysogeny broth , [ 1 ] but LB has also come to colloquially mean Luria broth , Lennox broth , life broth or Luria –Bertani medium . [ 2 ]
Lysogeny Broth. In 1948, around the time Bertani turned 25 years old, he immigrated to the United states as Carnegie Fellow. He was invited to work in a group with Milislav Demeric. He quickly shifted from his work in Drosophila genetics to become a pioneer in the field of microbial genetics.
Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2] It provides a source of amino acids for the growing bacteria .
This nutrient-rich microbial broth contains peptides, amino acids, water soluble vitamins and glucose in a low-salt formulation. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB medium (lysogeny broth). Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids.
Nutrient broth has the same composition, but lacks agar. [2] [3] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
Esther Miriam Zimmer Lederberg (December 18, 1922 – November 11, 2006) was an American microbiologist and a pioneer of bacterial genetics.She discovered the bacterial virus lambda phage and the bacterial fertility factor F, devised the first implementation of replica plating, and furthered the understanding of the transfer of genes between bacteria by specialized transduction.
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]
This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products. It was originally called M16 medium, [1] but in 1975 Terzaghi and Sandine [2] added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium.