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Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be located by a stain or other visualisation method after separation. [ 1 ]
used to separate particles dispersed in a liquid according to their molecular mass: Electrophoresis apparatus: used to detect and classify serum proteins or proteins from any other source; also used for DNA separation Chromatography: • Gas chromatography or Gas liquid chromatography (GLC) • Planar chromatography • Paper chromatography
Also used for DNA separation. Chemical laboratory apparatus: for Chemical tests: Chromatography: One of the basic modern "chemical examination" of body fluids and viscera;video links for details •Gas Chromatography or Gas Liquid Chromatography(GLC)-do- •Planar Chromatography-do- •Paper Chromatography-do- •Thin layer chromatography-do-
Paper chromatography is a technique that involves placing a small dot or line of sample solution onto a strip of chromatography paper. The paper is placed in a container with a shallow layer of solvent and sealed. As the solvent rises through the paper, it meets the sample mixture, which starts to travel up the paper with the solvent.
Chromatography separates dissolved substances by different interaction with (i.e., travel through) a material. High-performance liquid chromatography (HPLC) Thin-layer chromatography (TLC) Countercurrent chromatography (CCC) Droplet countercurrent chromatography (DCC) Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC)
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [ 1 ] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [ 2 ]
Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups. For reversed phase, alkyl hydrocarbons are the preferred stationary phase; octadecyl (C18) is the most common stationary phase, but octyl (C8) and butyl (C4) are also used in some applications. The designations for ...