Ads
related to: principles of paper chromatography
Search results
Results From The WOW.Com Content Network
Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.
They established the principles and basic techniques of partition chromatography, and their work encouraged the rapid development of several chromatographic methods: paper chromatography, gas chromatography, and what would become known as high-performance liquid chromatography. Since then, the technology has advanced rapidly.
LFTs derive from paper chromatography, which was developed in 1943 by Martin and Synge, [9] and elaborated in 1944 by Consden, Gordon and Martin. [10] [11] There was an explosion of activity in this field after 1945. [9]
Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC) ... Electrostatic separation, works on the principle of corona discharge, where two ...
Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [ 1 ] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [ 2 ]
In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In reversed phase the opposite is true; the stationary phase is nonpolar and the mobile phase is polar. Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups.
Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation technique of gas chromatography with the identification technique of mass spectrometry. GC-MS is the single most important analytical tool for the analysis of volatile and semi-volatile organic compounds in complex mixtures. [7]
Partition chromatography was one of the first kinds of chromatography that chemists developed, and is barely used these days. [25] The partition coefficient principle has been applied in paper chromatography, thin layer chromatography, gas phase and liquid–liquid separation applications.