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The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just below this point, only very specific base pairing between the primer and the template will occur.
Additionally, primer sequences need to be chosen to uniquely select for a region of DNA, avoiding the possibility of hybridization to a similar sequence nearby. A commonly used method for selecting a primer site is BLAST search, whereby all the possible regions to which a primer may bind can be seen. Both the nucleotide sequence as well as the ...
In these large cartridges a silk cord becket runs up the centre with a loop at the top for handling. [5] For quick-firing guns the charge is contained in a brass case to which is fitted a primer for igniting the charge. This case is inserted into the gun, and when fired slightly expands and tightly fits the chamber of the gun, thus acting as an ...
The experience was harrowing at times, like when she underwent grueling rounds of chemotherapy, (“My first round felt like a giant root canal,” she recalls.“My jaw hurt so bad like someone ...
The make-up of the university changed. Before the war the majority of lecturers were foreigners: now most of them were from Czech Crown lands. The number of students rose to 1,500 in 1727: in addition to locals there were many students from Hungary, Lusatia, Poland and Lithuania as well as from Russia. The Jesuit College building in 1724
Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the mRNA.
A water-based primer, used primarily on wood. A primer (/ ˈ p r aɪ m ər /) or undercoat is a preparatory coating put on materials before painting.Priming ensures better adhesion of paint to the surface, increases paint durability, and provides additional protection for the material being painted.
Detonating cord (also called detonation cord, detacord, detcord, blasting rope, or primer cord) is a thin, flexible plastic tube usually filled with pentaerythritol tetranitrate (PETN, pentrite). With the PETN exploding at a rate of approximately 6,400 m/s (21,000 ft/s), any common length of detonation cord appears to explode instantaneously.