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A vintage postcard with the United Shoe Machinery factory in Beverly, Massachusetts Share of the United Shoe Machinery Corporation, issued 4. May 1916. United Shoe Machinery Corporation (USMC) was a U.S.-based manufacturer of various industrial machinery, particularly for the shoe manufacturing industry and monopolized the American shoe machinery business. [1]
NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
These tools are used to optimize the design of primers for target DNA or cDNA sequences. Primer optimization has two goals: efficiency and selectivity. Efficiency involves taking into account such factors as GC-content, efficiency of binding, complementarity, secondary structure , and annealing and melting point (Tm) .
The PCR machine is heated in advance whilst the components are mixed over ice and then immediately placed into the PCR machine once it reaches optimum temperature. This would eliminate the warm-up process required, reduce non-specific annealing of the primers and ensures that any miss paired primers in the mixture are separated. [15] Freezing:
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
The fixed rate for a 15-year mortgage is 6.27%, up 13 basis points from last week's average 6.14%. These figures are lower than a year ago, when rates averaged 6.60% for a 30-year term and 5.76% ...
OTC Tools (originally Owatonna Tool Company) is a tool and equipment manufacturer. It was founded in Owatonna, Minnesota, by Godfrey Kaplan, who operated a small machine shop there. Kaplan's son Rueben, invented the "Grip-O-Matic" universal gear puller and received his first patent. [1] The tool remains virtually unchanged to this day. [2]
At lower temperatures, the primers bind less specifically. Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in early steps of amplification will "swamp out" any specific sequences because of the exponential nature of polymerase amplification.
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