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A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
A 40x magnification image of cells in a medical smear test taken through an optical microscope using a wet mount technique, placing the specimen on a glass slide and mixing with a salt solution Optical microscopy is used extensively in microelectronics, nanophysics, biotechnology, pharmaceutic research, mineralogy and microbiology.
A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens.
Oil-immersion objectives are used only at very large magnifications that require high resolving power. Objectives with high-power magnification have short focal lengths, facilitating the use of oil. The oil is applied to the specimen (conventional microscope), and the stage is raised, immersing the objective in oil. (In inverted microscopes the ...
In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by
Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...
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