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The E. Coli DnaG primase is a 581 residue monomeric protein with three functional domains, according to proteolysis studies. There is an N-terminal Zinc-binding domain (residues 1–110) where a zinc ion is tetrahedrally coordinated between one histidine and three cysteine residues, which plays a role in recognizing sequence specific DNA binding sites.
DNA primase is an enzyme involved in the replication of DNA and is a type of RNA polymerase.Primase catalyzes the synthesis of a short RNA (or DNA in some living organisms [1]) segment called a primer complementary to a ssDNA (single-stranded DNA) template.
Along the DNA template, primase intersperses RNA primers that DNA polymerase uses to synthesize DNA from in the 5′→3′ direction. [1] Another example of primers being used to enable DNA synthesis is reverse transcription. Reverse transcriptase is an enzyme that uses a template strand of RNA to synthesize a complementary strand of DNA.
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DnaA is a protein that activates initiation of DNA replication in bacteria. [1] Based on the Replicon Model, a positively active initiator molecule contacts with a particular spot on a circular chromosome called the replicator to start DNA replication. [2]
Research institutions are a subset of doctoral degree-granting institutions and conduct research. These institutions "conferred at least 20 research/scholarship doctorates in 2019-20 and reported at least $5 million in total research expenditures in FY20 were assigned to one of two categories based on a measure of research activity." [1]
College application is the process by which individuals apply to gain entry into a college or university.Although specific details vary by country and institution, applications generally require basic background information of the applicant, such as family background, and academic or qualifying exam details such as grade point average in secondary school and standardized testing scores.
[5] [6] [3] [7] The concept of prototyping in design disciplines' literature is also related to the concepts of experimentation (i.e., an iterative problem-solving process of trying, failing and improving), [4] and Research through Design (RtD) (i.e., designers make a prototype with the purpose of conducting research and generating knowledge ...