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It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. [1] Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and ...
Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium.It appears as a mucoid lactose fermenter on MacConkey agar.
English: MacConkey agar is a selective and differential medium used to isolate and differentiate Gram-negative bacteria, particularly those belonging to the family Enterobacteriaceae. It's primarily used to distinguish between lactose-fermenting (LF) and non-lactose-fermenting (NLF) colonies.
Non-fermenters (also non-fermenting bacteria) are a taxonomically heterogeneous group of bacteria of the phylum Pseudomonadota that cannot catabolize glucose, and are thus unable to ferment. This does not necessarily exclude that species can catabolize other sugars or have anaerobiosis like fermenting bacteria.
Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited. [2] Coliform organisms ferment the lactose in this medium, producing a green metallic sheen (i.e. Escherichia coli), whereas non-lactose-fermenting organisms produce clear, colourless colonies, [1] i.e. Salmonella species.
Yersinia enterocolitica is a Gram-negative, ... enterocolitica is non-lactose fermenting, and therefore show up on the plates as 2mm translucent pale colonies.
EMB is a differential microbiological media, which inhibits the growth of Gram-positive bacteria and differentiates bacteria that ferment lactose (e.g., E. coli) from those that do not (e.g., Salmonella, Shigella). [2] Organisms that ferment lactose appear dark/black or green often with "nucleated colonies"—colonies with dark centers. [3]
Burkholderia cepacia complex (BCC) is a species complex consisting of Burkholderia cepacia and at least 20 different biochemically similar species of Gram-negative bacteria. They are catalase -producing and lactose -non fermenting . [ 1 ]