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In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
Beginning the streak pattern. Label the base of the plate. Then, visualize the plate in four quadrants: top left (I), top right (II), bottom right (III), bottom left (IV). Streak the mixed culture back and forth in the first quadrant (top left) of the agar plate. Do not cut the agar, simply scrape the top. Flame the loop to rid of culture residue.
[1] [2] [4] The inoculation needle inoculates the sterile agar by the manipulation of the media so that the needle tip grazes the agar surface in a zigzag pattern. [1] Aseptic technique is then applied to the withdrawn inoculation needle. Plate culture. Inoculation of a plate culture is done through the streaking technique to make a streak plate.
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria.
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Negative selection through replica plating to screen for ampicillin sensitive colonies. Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary ...