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Electron microscope image of canine parvovirus isolated using buoyant density centrifugation. Buoyant density centrifugation (also isopycnic centrifugation or equilibrium density-gradient centrifugation) uses the concept of buoyancy to separate molecules in solution by their differences in density.
Centrifugal force separates components not only on the basis of density, but also of particle size and shape. In contrast, a more specialized equilibrium density-gradient centrifugation produces a separation profile dependent on particle-density alone, and therefore is suitable for more fine-grained separations.
Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this gradient is formed particles move within the gradient to the position having a density matching their own (this is in fact an incorrect description of the exact physical process but does describe the ...
The large particles reach their equilibrium density position early, while the small particles slowly migrate across the large particle zone and ultimately take up an equilibrium position deeper into the gradient. [18] A tube, after being centrifuged by this method, has particles in order of density based on height.
When DNA is extracted from these cells and made to undergo buoyant density centrifugation on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15 N medium had a higher density than cells grown in normal 14 N medium.
4.3 Density gradient centrifugation. 5 Data ... stoichiometry of non-covalent complexes and equilibrium constants of ... of this method scales approximately ...
In order to test hypotheses for how DNA replicates, Meselson and Stahl, together with Jerome Vinograd, invented a method that separates macromolecules according to their buoyant density. [6] The method, equilibrium density gradient centrifugation, was sufficiently sensitive that Meselson and Stahl were able to separate DNA containing the heavy ...
Concentration of Plasmodium falciparum-infected erythrocytes by discontinuous density gradient centrifugation in Percoll [1] Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, [2] and commercialized by Pharmacia Fine Chemicals. [3]