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KOH test on a vaginal wet mount, showing slings of pseudohyphae of Candida albicans surrounded by round vaginal epithelial cells, conferring a diagnosis of candidal vulvovaginitis. The KOH test , also known as a potassium hydroxide preparation or KOH prep , is a quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans ...
Vaginal wet mount showing slings of pseudohyphae of Candida albicans surrounded by round vaginal epithelial cells, conferring a diagnosis of candidal vulvovaginitis.. A vaginal wet mount (or vaginal smear [1] or wet prep [2]) is a gynecologic test wherein a sample of vaginal discharge is observed by wet mount microscopy by placing the specimen on a glass slide and mixing with a salt solution. [1]
There is an array of different tests to differentiate between fungi. Direct microscopy with 10% KOH would show small to medium conidia with ectothrix hair invasion. [11] Performing a wet mount would show 'racquet shaped hyphae' with few macro and microconidia. [11]
They have few septa and lack clamp connections. Fusiform skeletal hyphae are the second form of skeletal hyphae. Unlike typical skeletal hyphae these are swollen centrally and often exceedingly broad, hence giving the hypha a fusiform shape. Binding hyphae are thick-walled and frequent branched. Often they resemble deer antlers or defoliated ...
The reagent for the K test is an aqueous solution of potassium hydroxide (KOH) (10–25%), or, in the absence of KOH, a 10% aqueous solution of sodium hydroxide (NaOH, lye), which provides nearly identical results. [4] A 10% solution of KOH will retain its effectiveness for about 6 months to a year. [5]
Gram stain of Candida albicans from a vaginal swab, showing hyphae, and chlamydospores, which are 2–4 μm in diameter.. A chlamydospore is the thick-walled large resting spore of several kinds of fungi, including Ascomycota such as Candida, [1] Basidiomycota such as Panus, [2] and various Mortierellales species. [3]
The study saw that out of 150 patients CFW showed positive in 95 of them. The study also reviewed key advantages for using the Calcofluor-White stain over KOH. It discussed that CFW requires a quick screening time as it can be seen on low power objectives and can be done within a minute while KOH requires about 3–4 minutes for each slide.
Malassezia furfur is a fungus that lives on the superficial layers of the dermis.It generally exists as a commensal organism forming a natural part of the human skin microbiota, but it can gain pathogenic capabilities when morphing from a yeast to a hyphal form during its life cycle, through unknown molecular changes. [2]