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Digital slides can be retrieved from a storage system, and viewed on a computer screen, by running image management software on a standard web browser, and assessed in exactly the same way as on a microscope. [1] Digital slides can be used as an alternative to traditional viewing for the purpose of teleconsultation. [2]
A microscopy slide scanner. Whole slide image quality comparison, with a slide scanned with a 20x objective and about 0.8 gigabytes (GB) in size to the left, and a 40x objective and approximately 1.2 GB in size to the right. Each image shows a red blood cell. Digital slides are created from glass slides using specialized scanning machines.
Prior to recent advances in virtual microscopy, slides were commonly digitized by various forms of film scanner and image resolutions rarely exceeded 5000 dpi. Nowadays, it is possible to achieve more than 100,000 dpi and thus resolutions approaching that visible under the optical microscope .
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A flying-spot scanner (FSS) uses a scanning source of a spot of light, such as a high-resolution, high-light-output, low-persistence cathode ray tube (CRT), to scan an image. Usually the image to be scanned is on photographic film, such as motion picture film, or a slide or photographic plate. The output of the scanner is usually a television ...
Some slide films can have a Dmax close to 4.0d with proper exposure, and so can black-and-white negative film. Consumer-level flatbed photo scanners have a dynamic range in the 2.0–3.0 range, which can be inadequate for scanning all types of photographic film, as Dmax can be and often is between 3.0d and 4.0d with traditional black-and-white ...