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A complete cycle involves heating the oven to the required temperature, maintaining that temperature for the proper time interval for that temperature, turning the machine off and cooling the articles in the closed oven till they reach room temperature. The standard settings for a hot air oven are: 1.5 to 2 hours at 160 °C (320 °F)
Hot air oven: used in sterilizing instruments for various aseptic procedures, specially if that can not be autoclaved like powders Koch's or Arnold's steam sterilizer: used for steam sterilization A pressure cooker: used as a portable autoclave Biological and chemical indicators
A mechanical convection oven contains a blower that actively forces heated air throughout all areas of the chamber. The flow created by the blower ensures uniform temperatures and the equal transfer of heat throughout the load. For this reason, the mechanical convection oven is the more efficient of the two processes.
Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]
Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [ 1 ] [ 2 ] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores , which can germinate shortly thereafter and resume growth.
Other forms of the laboratory oven include vacuum ovens, forced air convection ovens, and gravity convection ovens. [1] Forensic labs use vacuum ovens that have been configured in specific ways to assist in developing fingerprints. Gravity convection ovens are used for biological purposes such as removing biological contaminants from samples ...
historically used for anaerobiosis; a lit candle was placed in as air-tight jar such that when it went out it would be because it used up all the available oxygen: Castaneda's medium / Castaneda's bottle: used for simultaneous solid and liquid cultures in many bottles Centrifuge: to separate supernatant & pellet Cragie tube: see link: Desiccator
Preparation of microbiological samples in a laminar chamber. A laminar flow cabinet or tissue culture hood is a partially enclosed bench work surface designed to prevent contamination of biological samples, semiconductor wafer, or any particle-sensitive materials.