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Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
Introgression or introgressive hybridization is the incorporation (usually via hybridization and backcrossing) of novel genes or alleles from one taxon into the gene pool of a second, distinct taxon.
Generally, hybridization is more frequently observed in species with external fertilization including plants but also fishes, than in internally fertilized clades. [4] In plants, high rates of selfing in some species may prevent hybridization, and breeding system may also affect the frequency of heterospecific pollen transfer.
Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence . [ 1 ]
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
While Hybridization and breeding methods have produced successful crop species, declining yield is a major challenge. [ editorializing ] Thus, further research is needed for leveraging hybridization in perennial crop systems to produce sustainable and high yielding crops.
Introgressive hybridization, also known as introgression, is the flow of genetic material between divergent lineages via repeated backcrossing. In plants, this backcrossing occurs when an F 1 {\displaystyle F_{1}} generation hybrid breeds with one or both of its parental species.
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...